Electrochemical DNA Sensor Using Genetically Engineered Thermostable Pyrroloquinoline Quinone Glucose Dehydrogenase
نویسندگان
چکیده
منابع مشابه
A Disposable Electrochemical Glucose Sensor Using Catalytic Subunit of Novel Thermostable Glucose Dehydrogenase
We report here the development of a novel disposable-type glucose enzyme sensor based on a screen-printed carbon electrode (SPCE) employing a catalytic subunit of FAD-dependent glucose dehydrogenase (FADGDH) and a Rucomplex (hexaammineruthenium (III) chloride) as the electron mediator. The whole blood glucose measurement was achieved in 2 seconds using a 500 nl sample of the range of 100-800 mg...
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An organically modified sol-gel glass (ORMOSIL) encapsulating pyrroloquinoline quinone (PQQ)-modified electrode for the rapid, sensitive and simple determination of thiol-containing compounds such as cysteine and glutathione is reported. The effect of applied potential, nature of thiol compound and pH on the response of the sensor was examined and optimum conditions were determined. The electro...
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We have developed a novel method, antagonistic template-based biopanning, for screening peptide ligands specifically recognizing local tertiary protein structures. We chose water-soluble pyrroloquinoline quinone (PQQ) glucose dehydrogenase (GDH-B) as a model enzyme for this screening. Two GDH-B mutants were constructed as antagonistic templates; these have some point mutations to induce disrupt...
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An engineered Escherichia coli PQQ glucose dehydrogenase (PQQGDH) with improved enzymatic characteristics was constructed by substituting and combining the gene-encoding protein regions responsible for EDTA tolerance, thermal stability and substrate specificity. The protein region responsible for complete EDTA tolerance in Acinetobacter calcoaceticus, which is recognized as the indicator of hig...
متن کاملStructure of the pyrroloquinoline quinone radical in quinoprotein ethanol dehydrogenase.
Quinoprotein alcohol dehydrogenases use the pyrroloquinoline quinone (PQQ) cofactor to catalyze the oxidation of alcohols. The catalytic cycle is thought to involve a hydride transfer from the alcohol to the oxidized PQQ, resulting in the generation of aldehyde and reduced PQQ. Reoxidation of the cofactor by cytochrome proceeds in two sequential steps via the PQQ radical. We have used a combina...
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ژورنال
عنوان ژورنال: Electrochemistry
سال: 2003
ISSN: 1344-3542,2186-2451
DOI: 10.5796/electrochemistry.71.490